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inf2 c terminal  (Proteintech)


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    Structured Review

    Proteintech inf2 c terminal
    Inf2 C Terminal, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+inf2/INF2+Antibody/pmc12569161-15-0-3
    Average 94 stars, based on 49 article reviews
    inf2 c terminal - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Targeting INF2 with DiosMetin 7-O-β-D-Glucuronide: a new stratagem for colorectal cancer therapy
    Article Snippet: .. NC si1 si2 si3 35 kDa 25 kDa 250 kDa SW620 150 kDa 100 kDa 70 kDa 50 kDa 40 kDa Fig. S3 Western blot membrane of INF2 (180-200 kDa) protein detected with anti-INF2 (1:2000, Cat. No. 20466-1-AP, Proteintech, Wuhan, China) antibody. ..

    Article Title: Targeting INF2 with DiosMetin 7-O-β-D-Glucuronide: a new stratagem for colorectal cancer therapy
    Article Snippet: .. 70 kDa 50 kDa 40 kDa 35 kDa 25 kDa 100 kDa SW480Alginic acid 46 49 52 55 58 61 46 49 52 55 58 61 150 kDa 100 kDa 70 kDa 50 kDa 40 kDa Fig. S11 Western blot membrane of INF2 (180-200 kDa) protein detected with anti-INF2 (1:2000, Cat. No. 20466-1-AP, Proteintech, Wuhan, China) antibody. ..

    Article Title: Targeting INF2 with DiosMetin 7-O-β-D-Glucuronide: a new stratagem for colorectal cancer therapy
    Article Snippet: .. SW480Uridine 5′-diphosphoglucose 70 kDa 50 kDa 40 kDa 35 kDa 25 kDa 100 kDa 46 49 52 55 58 61 SW480Alginic acid Fig. S9 Western blot membrane of INF2 (180-200 kDa) protein detected with anti-INF2 (1:2000, Cat. No. 20466-1-AP, Proteintech, Wuhan, China) antibody. ..

    Article Title: Targeting INF2 with DiosMetin 7-O-β-D-Glucuronide: a new stratagem for colorectal cancer therapy
    Article Snippet: .. 250 kDa SW480 150 kDa 100 kDa 70 kDa 50 kDa 40 kDa Fig. S1 Western blot membrane of INF2 (180-200 kDa) protein detected with anti-INF2 (1:2000, Cat. No. 20466-1-AP, Proteintech, Wuhan, China) antibody. .. Gel (10%)-separated proteins were transferred to nitrocellulose membranes (0.2 um pore size; Thermo Fisher Scientific, USA) by wet electroblotting (310 mA, 120 min).

    Article Title: Targeting INF2 with DiosMetin 7-O-β-D-Glucuronide: a new stratagem for colorectal cancer therapy
    Article Snippet: .. 70 kDa 50 kDa 40 kDa 35 kDa 25 kDa 100 kDa SW480-DMSO 46 49 52 55 58 61 SW480Uridine 5′-diphosphoglucose Fig. S7 Western blot membrane of INF2 (180-200 kDa) protein detected with anti-INF2 (1:2000, Cat. No. 20466-1-AP, Proteintech, Wuhan, China) antibody. ..

    Article Title: Targeting INF2 with DiosMetin 7-O-β-D-Glucuronide: a new stratagem for colorectal cancer therapy
    Article Snippet: .. Fig. S5 Western blot membrane of INF2 (180-200 kDa) protein detected with anti-INF2 (1:2000, Cat. No. 20466-1-AP, Proteintech, Wuhan, China) antibody. .. Gel (7.5%)-separated proteins were transferred to nitrocellulose membranes (0.2 um pore size; Thermo Fisher Scientific, USA) by wet electroblotting (310 mA, 120 min).

    Membrane:

    Article Title: Targeting INF2 with DiosMetin 7-O-β-D-Glucuronide: a new stratagem for colorectal cancer therapy
    Article Snippet: .. NC si1 si2 si3 35 kDa 25 kDa 250 kDa SW620 150 kDa 100 kDa 70 kDa 50 kDa 40 kDa Fig. S3 Western blot membrane of INF2 (180-200 kDa) protein detected with anti-INF2 (1:2000, Cat. No. 20466-1-AP, Proteintech, Wuhan, China) antibody. ..

    Article Title: Targeting INF2 with DiosMetin 7-O-β-D-Glucuronide: a new stratagem for colorectal cancer therapy
    Article Snippet: .. 70 kDa 50 kDa 40 kDa 35 kDa 25 kDa 100 kDa SW480Alginic acid 46 49 52 55 58 61 46 49 52 55 58 61 150 kDa 100 kDa 70 kDa 50 kDa 40 kDa Fig. S11 Western blot membrane of INF2 (180-200 kDa) protein detected with anti-INF2 (1:2000, Cat. No. 20466-1-AP, Proteintech, Wuhan, China) antibody. ..

    Article Title: Targeting INF2 with DiosMetin 7-O-β-D-Glucuronide: a new stratagem for colorectal cancer therapy
    Article Snippet: .. SW480Uridine 5′-diphosphoglucose 70 kDa 50 kDa 40 kDa 35 kDa 25 kDa 100 kDa 46 49 52 55 58 61 SW480Alginic acid Fig. S9 Western blot membrane of INF2 (180-200 kDa) protein detected with anti-INF2 (1:2000, Cat. No. 20466-1-AP, Proteintech, Wuhan, China) antibody. ..

    Article Title: Targeting INF2 with DiosMetin 7-O-β-D-Glucuronide: a new stratagem for colorectal cancer therapy
    Article Snippet: .. 250 kDa SW480 150 kDa 100 kDa 70 kDa 50 kDa 40 kDa Fig. S1 Western blot membrane of INF2 (180-200 kDa) protein detected with anti-INF2 (1:2000, Cat. No. 20466-1-AP, Proteintech, Wuhan, China) antibody. .. Gel (10%)-separated proteins were transferred to nitrocellulose membranes (0.2 um pore size; Thermo Fisher Scientific, USA) by wet electroblotting (310 mA, 120 min).

    Article Title: Targeting INF2 with DiosMetin 7-O-β-D-Glucuronide: a new stratagem for colorectal cancer therapy
    Article Snippet: .. 70 kDa 50 kDa 40 kDa 35 kDa 25 kDa 100 kDa SW480-DMSO 46 49 52 55 58 61 SW480Uridine 5′-diphosphoglucose Fig. S7 Western blot membrane of INF2 (180-200 kDa) protein detected with anti-INF2 (1:2000, Cat. No. 20466-1-AP, Proteintech, Wuhan, China) antibody. ..

    Article Title: Targeting INF2 with DiosMetin 7-O-β-D-Glucuronide: a new stratagem for colorectal cancer therapy
    Article Snippet: .. Fig. S5 Western blot membrane of INF2 (180-200 kDa) protein detected with anti-INF2 (1:2000, Cat. No. 20466-1-AP, Proteintech, Wuhan, China) antibody. .. Gel (7.5%)-separated proteins were transferred to nitrocellulose membranes (0.2 um pore size; Thermo Fisher Scientific, USA) by wet electroblotting (310 mA, 120 min).



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    ( A ) Western blot for VAP-B and myc-tag showing VAP-B expression in control and myc-VAP-B overexpressed U2OS cells. <t>INF2</t> is used as loading control. Molecular weight in kDa. ( B ) Quantification of CCCP-induced actin polymerization in control and myc-VAP-B-overexpressing U2OS cells. Data with 65 cells (control) and 53 cells (overexpressing myc-VAP-B) from 3 independent runs. Error ± SEM. ( C ) Averaged traces showing mitochondrial calcium fold change (MitoGCamp6f) following histamine stimulation (at time 0) in control (ER-RFP expressing), ER-RFP-mito (ER-Mito linker) and PM-RFP-Mito (PM-Mito-linker) transfected HeLa cells. Data from 2 independent experiments comprising of 4 individual traces for each condition. Error: ±SEM. ( D ) Dot plot of peak fold change in mitochondrial calcium following histamine stimulation from individual traces as in Fig S4C. Data for 32 cells (Control- ER-RFP); 32 cells (ER-RFP-Mito); 28 cells (PM-RFP-Mito) for 3 independent runs. Error: ±s.d. P = 0.0213 (*) for Control Vs ER-RFP-Mito, P = 0.0439 (*) for Control Vs PM-RFP-Mito and P = 0.0001 (****) for ER-RFP-Mito Vs PM-RFP-Mito, One-way ANOVA used. ( E ) Graph showing actin filaments intensity (GFP-Ftractin) from HeLa cells transiently expressing either ER-RFP-Mito (magenta) or PM-RFP-Mito or ER-RFP, treated with 20 µM CCCP. Data from 28 cells (ER-RFP); 27 cells (ER-RFP-Mito) and 23 cells (PM-RFP-Mito) from 4 independent experiments. Error: ±SEM.
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    Image Search Results


    ( A ) Western blot for VAP-B and myc-tag showing VAP-B expression in control and myc-VAP-B overexpressed U2OS cells. INF2 is used as loading control. Molecular weight in kDa. ( B ) Quantification of CCCP-induced actin polymerization in control and myc-VAP-B-overexpressing U2OS cells. Data with 65 cells (control) and 53 cells (overexpressing myc-VAP-B) from 3 independent runs. Error ± SEM. ( C ) Averaged traces showing mitochondrial calcium fold change (MitoGCamp6f) following histamine stimulation (at time 0) in control (ER-RFP expressing), ER-RFP-mito (ER-Mito linker) and PM-RFP-Mito (PM-Mito-linker) transfected HeLa cells. Data from 2 independent experiments comprising of 4 individual traces for each condition. Error: ±SEM. ( D ) Dot plot of peak fold change in mitochondrial calcium following histamine stimulation from individual traces as in Fig S4C. Data for 32 cells (Control- ER-RFP); 32 cells (ER-RFP-Mito); 28 cells (PM-RFP-Mito) for 3 independent runs. Error: ±s.d. P = 0.0213 (*) for Control Vs ER-RFP-Mito, P = 0.0439 (*) for Control Vs PM-RFP-Mito and P = 0.0001 (****) for ER-RFP-Mito Vs PM-RFP-Mito, One-way ANOVA used. ( E ) Graph showing actin filaments intensity (GFP-Ftractin) from HeLa cells transiently expressing either ER-RFP-Mito (magenta) or PM-RFP-Mito or ER-RFP, treated with 20 µM CCCP. Data from 28 cells (ER-RFP); 27 cells (ER-RFP-Mito) and 23 cells (PM-RFP-Mito) from 4 independent experiments. Error: ±SEM.

    Journal: EMBO Reports

    Article Title: Peri-mitochondrial actin filaments inhibit Parkin assembly by disrupting ER-mitochondria contacts

    doi: 10.1038/s44319-025-00561-y

    Figure Lengend Snippet: ( A ) Western blot for VAP-B and myc-tag showing VAP-B expression in control and myc-VAP-B overexpressed U2OS cells. INF2 is used as loading control. Molecular weight in kDa. ( B ) Quantification of CCCP-induced actin polymerization in control and myc-VAP-B-overexpressing U2OS cells. Data with 65 cells (control) and 53 cells (overexpressing myc-VAP-B) from 3 independent runs. Error ± SEM. ( C ) Averaged traces showing mitochondrial calcium fold change (MitoGCamp6f) following histamine stimulation (at time 0) in control (ER-RFP expressing), ER-RFP-mito (ER-Mito linker) and PM-RFP-Mito (PM-Mito-linker) transfected HeLa cells. Data from 2 independent experiments comprising of 4 individual traces for each condition. Error: ±SEM. ( D ) Dot plot of peak fold change in mitochondrial calcium following histamine stimulation from individual traces as in Fig S4C. Data for 32 cells (Control- ER-RFP); 32 cells (ER-RFP-Mito); 28 cells (PM-RFP-Mito) for 3 independent runs. Error: ±s.d. P = 0.0213 (*) for Control Vs ER-RFP-Mito, P = 0.0439 (*) for Control Vs PM-RFP-Mito and P = 0.0001 (****) for ER-RFP-Mito Vs PM-RFP-Mito, One-way ANOVA used. ( E ) Graph showing actin filaments intensity (GFP-Ftractin) from HeLa cells transiently expressing either ER-RFP-Mito (magenta) or PM-RFP-Mito or ER-RFP, treated with 20 µM CCCP. Data from 28 cells (ER-RFP); 27 cells (ER-RFP-Mito) and 23 cells (PM-RFP-Mito) from 4 independent experiments. Error: ±SEM.

    Article Snippet: Primary antibody: anti-myc-tag antibody (Abcam, ab32 used 1:1000) anti-INF2 antibody (CST #41081; used at 1:1000) anti-VAP-B antibody (Proteintech #14477-1-AP; used at 1:5000 at RT) anti-LC3B antibody (CST #12741S; used at 1:1000) anti-Parkin antibody (CST #2132S; used at 1:1000) anti-Parkin(phospho S65) antibody (Abcam #315376; used at 1:1000) anti-Pink1 antibody (CST #6946S; used at 1:1000) anti-β-actin antibody (CST #3700S; used at 1:10,000) anti-Mfn2 antibody (CST #9482S; used at 1:1000) anti-VDAC antibody (Invitrogen #MA5-41088; used at 1:1000) anti-Phospho ubiquitin (Ser65) (CST #62802S; used at 1:1000).

    Techniques: Western Blot, Expressing, Control, Molecular Weight, Transfection